Welcome to the Vector and Waterborne Pathogens Research Group NGS Analysis page.



This webpage was created me (Siobhon Egan) during an internship program at Pawsey Supercomputing Centre provided by CSIRO in 2017/2018.

It takes you through analysis of bacterial 16S amplicon next-generation sequencing data - however it can be easily adapted to suit various other amplicon sequence data.

As with anything coding and bioinformatics related this is just one method of data analysis. The internet is wealth of information on programs, coding languages, plug ins and a number of different GUIs which you can use.

The aim of this webpage is to help by “taming the flood” of what can seem like a never ending world of NGS data analysis. I have endevroued to credit the resource and works of other people where appropriate - but if you feel your work has not been credited please send an email to

For more information on the research group head to our website.

I also maintain some resources on my personal github page, or see my website.



Vector and Waterborne Pathogens Research Group. 2018. S Egan.